Fig. 3

Cirsiliol affects the mitochondrial membrane potential of osteosarcoma cells. A, B U2OS and U2OS/MTX cells were treated with DMSO (20 µM), respectively, and incubated with JC-1 for 30 min. Representative confocal microscopy images. Scale bar: 100 µm. C U2OS and U2OS/MTX cells were treated with DMSO (20 µM), respectively, and incubated with JC-1 for 30 min. The distribution of JC-1 aggregates and monomers was examined by flow cytometry. D Flow cytometry analysis of JC-1 monomers and JC-1 aggregates at different concentrations. E Statistical plot of the difference in mitochondrial membrane potential between U2OS and U2OS/MTX cells under the same concentration of cirsiliol treatment. F Western blot analysis of lysates from cirsiliol-treated U2OS and U2OS/MTX cells against Cyto-C, MFN2 and ACTB antibodies. Data are expressed as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001