Fig. 1

Cirsiliol inhibits cell proliferation and cell cycle in OS. A Cell proliferation was measured using the CCK-8 assay. U2OS and U2OS/MTX cells were treated with different concentrations of cirsiliol (0, 5, 10, 20 µM) for 72 h and absorbance values were measured at 450 nm for each group. B A CCK-8 assay was performed to assess the viability of U2OS/MTX cells treated with MTX and cirsiliol at each designated concentration for 48 h. C, D. U2OS and U2OS/MTX cells were treated with cirsiliol (0, 10, 20 µM) to measure the expression of proliferating DNA. Three independent experiments were performed. Scale bars: 100 µm. E. Under the same concentration of cirsiliol treatment, a statistical plot of the difference in cell proliferation between U2OS and U2OS/MTX cells was shown. F U2OS and U2OS/MTX cells were treated with DMSO (20 µM) for 48 h. The distribution of cell cycles was measured using flow cytometry. Data are expressed as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001