Fig. 7
From: SIX1 amplification modulates stemness and tumorigenesis in breast cancer
In vitro alterations in tumor growth rate, stem cell marker expression and tumorigenic capacity in response to modulation of SIX1 expression levels. A Luminescence imaging of 66cl4-SCR-luc, 66cl4-shSix1 KD1-luc, and 66cl4-shSix1 KD2-luc tumor-bearing mice on week 1, 2, and 3 (n = 6); B Photograph of dissected tumors (n = 6); C Quantitation of luminescent signal of 66cl4-SCR-luc, 66cl4-shSix1 KD1-luc, and 66cl4-shSix1 KD2-luc tumor-bearing mice (n = 6); D Tumor growth curves of 66cl4-SCR-luc, 66cl4-shSix1 KD1-luc, and 66cl4-shSix1 KD2-luc tumor-bearing mice (n = 6). E–I Representative immunofluorescent images of Oct4, Sox2, Aldh1a1, Epcam, and Itgb1 in tumor tissues generated by 66cl4-SCR-luc, 66cl4-shSix1 KD1-luc, and 66cl4-shSix1 KD2-luc cells. J Tumor formation rates of BALB/C mice at 6–8 weeks after injecting different amounts of 66cl4-SCR-luc, 66cl4-shSix1 KD1-luc, and 66cl4-shSix1 KD2-luc cells into the fat pad of BALB/C mice for 10 days are shown in a table (n = 4–8). K Tumor volumes at the end of the experiment are presented (n = 4–8). L Tumor weights at the end of the experiment are provided (n = 4–8). M Tumors harvested at the end of the experiment are depicted (n = 4–8)