Fig. 5
From: SIX1 amplification modulates stemness and tumorigenesis in breast cancer
In vitro alterations in breast cancer stem cell associated markers and corresponding changes in the STAT3 signaling pathway in response to modulation of SIX1 expression levels. A, B and H Representative western blot analysis of whole cell lysates from 66cl4-SCR, 66cl4-shSIX1 KD1, 66cl4-shSIX1 KD2, MCF-7-NC and MCF-7-SIX1 cells. C–G qRT-PCR analyses of SIX1 and breast cancer stem cell associated markers using 1 μg RNA from 66cl4-SCR, 66cl4-shSIX1 KD1, 66cl4-shSIX1 KD2, MCF-7-NC and MCF-7-SIX1 cells. Gene expression is normalized to β-actin mRNA (n = 3)