Fig. 3

The mRNA and protein landscape of SIX1. A The volcano plot was constructed using the fold change values and P-adjust. Red dots indicate upregulated genes; blue dots indicate downregulated genes; grey dots indicate not significant. B Protein–protein interaction network revealed the interactions among proteins translated from differentially expressed genes, identified through screening based on SIX1 expression levels. C and D GO analysis of potential targets of mRNAs, the biological process, cellular component, and molecular function of potential targets were clustered based on ClusterProfiler package in R software (version: 3.18.0). The enriched KEGG signaling pathways were selected to demonstrate the primary biological actions of major potential mRNA. The abscissa indicates gene ratio and the enriched pathways were presented in the ordinate. Colors represent the significance of differential enrichment, the size of the circles represents the number of genes analyzed, the larger the circle, the greater the number of genes. In the enrichment result, P < 0.05 or FDR < 0.05 is considered to be a meaningful pathway (enrichment score with − log10 (P) of more than 1.3)