Fig. 1

The expression of SIX1 and its association with the clinical prognosis of breast cancer patients. A Differential expression of SIX1 across different cancer types based on pan-cancer analysis. B Box plots illustrating expression analysis of SIX1 in four major subclasses of breast cancer patients. C–F Kaplan–Meier survival curves of overall survival (OS) based on SIX1 expression in the four major subclasses of breast cancer patients (log-rank test, P < 0.01). G–I Box plots depicting SIX1 expression analysis according to breast cancer histology, stages, and nodal metastasis, respectively. J Scatter plot demonstrating the correlation between SIX1 expression and ploidy in each tumor, with positive correlation shown in red and negative correlation shown in blue. K Correlation scatter plot indicating the relationship between SIX1 expression and stromal score and immune score in breast cancer. A value > 0 represents a positive correlation, while an r < 0 represents a negative correlation. L Heat map displaying the correlation between SIX1 expression and immune cell infiltration in breast cancer patients. The square above each cell type is divided into two triangles. The color of the top left triangle represents the correlation, where red indicates positive correlation and blue indicates negative correlation. The color of the bottom right triangle represents statistical significance, with lighter colors indicating greater statistical significance. *P < 0.05, **P < 0.01, ***P < 0.001