Fig. 7

Klf10 downregulation inhibits chondrocyte extracellular matrix calcification and senescence, attenuating articular cartilage degeneration in DMM mice. A Schematic of mouse DMM model construction and lentivirus intra-articular injection. B, C Representative images of Safranin O/Fast Green staining of mouse knee cartilage. The degeneration of articular cartilage in different treatment groups was evaluated using the OARSI score. Scale bar: 100 μm, 50 μm. D–G Micro-CT was used to detect the knee joints of mice after DMM or sham, and SKY Scan software was used to analyze osteophyte volume, bone density and bone volume fraction (n = 5). H, I Alizarin red was used to stain the knee sections without decalcification (n = 5)