Fig. 6

Restoration of Fzd9 expression aggravates chondrocyte extracellular matrix calcification, and recovery of calcification aggravates chondrocyte senescence. A, B Murine articular chondrocytes were treated with CPPD. The expressions of P16, P21, Mmp13 and Col2a1 were detected by western blot (n = 3). C, D, G, H β-Galactosidase staining was used to show senescent chondrocytes, and image J was used to calculate proportions (n = 3). Scale bar: 100 μm. E, F Murine articular chondrocytes were treated with BCP. The expressions of P16, P21, Mmp13 and Col2a1 were detected by western blot (n = 3)