Fig. 5

Restoration of FZD9 expression aggravates chondrocyte extracellular matrix calcification, and recovery of calcification aggravates chondrocyte senescence. A, B Alizarin red dye shows the ability of overexpression treated chondrocytes to form calcified nodules (n = 3). Scale bar: 100 μm. C The alkaline phosphatase kit was used to measure the alkaline phosphatase activity of chondrocytes (n = 3). D, E Relative calcification related gene mRNA expressions of murine chondrocytes were detected using RT-qPCR (n = 3). F Murine articular chondrocytes were treated with lentivirus. The expressions of P16, P21 and Mmp13 were detected by western blot