Fig. 3

Klf10 downregulation affects calcium ion content but not phosphate ion. A Q-PCR was used to detect the expression of related genes (n = 3). B The ATP test kit is used to detect ATP produced by articular cartilage cells (n = 3). C–G Confocal microscopy of cells treated with Fluo-8 dye. The red arrows are positive cells. Data analysis using Zeiss software ZEN (n = 3). Scale bar:100 μm. H, I Relative gene mRNA expressions of murine chondrocytes were detected using RT-qPCR (n = 3)