Fig. 2

Klf10 knockdown attenuates TBHP induced chondrocytes extracellular matrix calcification. A, B Chondrocytes were treated with TBHP and siRNA (Klf10) and its negative control, and alizarin red staining was used to detect the formation of calcium nodules (n = 3). Scale bar: 100 μm. C The alkaline phosphatase kit was used to measure the alkaline phosphatase activity of chondrocytes (n = 3). D, E Relative calcification related gene mRNA expressions of murine chondrocytes were detected using RT-qPCR (n = 3)