Fig. 1

Extracellular matrix calcification occurs in senescence chondrocytes and OA cartilage. A, B Mouse articular chondrocytes were treated with different concentrations of TBHP, alizarin red dye was used to show the size of calcium nodules (n = 3). Scale bar: 100 μm. C The alkaline phosphatase kit was used to measure the alkaline phosphatase activity of chondrocytes treated with TBHP at different concentrations (n = 3). D–G Micro-CT was used to detect the knee joints of mice after DMM 2 and 8 weeks, and SKYscan software was used to analyze osteophyte volume, bone density and bone volume fraction (n = 5). H, I Alizarin red was used to stain the knee sections without decalcification. The black line area represented the calcified cartilage area (n = 5). Scale bar: 20 μm