Fig. 8

Further validation of miR-760-3p/Adcy1 and related cGMP/PKG and Apelin pathways in adult retinas with high myopia and their potential relationship with sclera remodeling. A The relative expressions of miR-760-3p were measured by qRT-PCR in human retinas (n = 3). B The relative expressions of Adcy1 mRNA were measured by qRT-PCR in human retinas (n = 3). C The targeting relationship between miR-760 and Adcy1 was predicted. D–G Western blot analysis of cGMP protein expression (D, E), PKG protein expression (D, F) and HIF-1a protein expression (D, G) in human retinas (n = 3). H–J Western blot analysis of MMP-2 protein expression (H, I) and p-ERK/t-ERK protein expression (H, J) in sclera from human (n = 3). K Morphological observation with H&E-stained retinas in human (scale bar: 50 µm). L Images of c-GMP (brown) immunohistochemistry in retina from human (scale bar: 20 µm). M Images of Adcy1 (red) immunofluorescence in retina from human with nucleus (blue) (scale bar: 20 µm). N Images of Apelin (green) immunofluorescence in retina from human with nucleus (blue) (scale bar: 20 µm). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. HC adults: healthy control adults; HP adults: high myopia adults