Fig. 7

Further validation of potential regulatory cGMP/PKG and Apelin pathways of miR-760-3p/Adcy1 in FDM mouse retinas its potential relationship with sclera remodeling. A Images of cGMP (red) immunofluorescence in retina from mice with nucleus (blue) (scale bar: 20 µm). B Images of Apelin (green) immunofluorescence in retina from mice with nucleus (blue) (scale bar: 20 µm). C, D IOD analysis of cGMP expression (C) and Apelin (D) in RGCs from mice. (E) The relative expressions of Apelin mRNA in retina were measured by qRT-PCR. F–H Western blot analysis of PKG protein expression (F, G) and HIF-1a protein expression (F, H) in mouse retinas. I–K Western blot analysis of MMP-2 protein expression (I, J) and p-ERK/t-ERK protein expression (I, K) in sclera from mice. For each group, n = 3–5. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. NCG: normal control group; FDMG fellow: form-deprived myopia fellow control group; FDMG: form-deprived myopia group