Fig. 5

Analysis of related regulatory cGMP/PKG and apelin pathways of lncRNA-XR_002792574.1/miR-760-3p/Adcy1 axis in FDM guinea pig RGCs and its potential relationship with sclera remodeling. A Images of cGMP (red) immunofluorescence in retina from guinea pigs with nucleus (blue) (scale bar: 20 µm). B Images of Apelin (green) immunofluorescence in retina from guinea pigs with nucleus (blue) (scale bar: 20 µm). C, D IOD analysis of cGMP expression (C) and Apelin (D) in RGCs from guinea pig. E–G Western blot analysis of PKG protein expression (E, F) and HIF-1a protein expression (E, G) in guinea pig RGCs. H–J Western blot analysis of MMP-2 protein expression (H, I) and p-ERK/t-ERK protein expression (H, J) in sclera from guinea pigs. For each group, n = 3–6. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. NCG: normal control group; FDMG fellow: form-deprived myopia fellow control group; FDMG: form-deprived myopia group