Fig. 4

Functional analysis of ceRNA network and validations of differential RNA expression of ceRNA network in guinea pigs. A Top 10 enriched GO terms of enriched mRNA target genes in ceRNA networks. B Top 30 KEGG enrichment analysis of enriched mRNA target genes in ceRNA networks. C–E The key lncRNA expressions of lncRNA-XR_001202354.2, lncRNA-XR_002792574.1 and lncRNA-XR_001199906.2 were measured by qRT-PCR. F–I The relative expressions of miR-532-3p, miR-342-5p, miR-760-3p and miR-574-5p were measured by qRT-PCR. J–L The relative mRNA expressions of Elfn2, Xkr7 and Adcy1 were measured by qRT-PCR. M Images of Adcy1 (brown) immunohistochemistry in retina from guinea pigs (scale bar: 20 µm). N IOD analysis of Adcy1 expression in GCL from guinea pig. O The targeting relationship between lncRNA-XR_002792574.1 and miR-760-3p was predicted. P The targeting relationship between miR-760-3p and Adcy1 was predicted. For each group, n = 4–6. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. NCG: normal control group; FDMG fellow: form-deprived myopia fellow control group; FDMG: form-deprived myopia group