Fig. 4
From: Parathyroid hormone stimulates bone regeneration in an atrophic non-union model in aged mice
A Representative immunohistochemical images of CD31-positive microvessels (arrowheads) within the callus tissue of controls and PTH-treated mice at 2 and 10 weeks after surgery. Scale bars: 25 μm. B CD31-positive microvessels/HPF within the callus tissue of controls (white bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) and PTH-treated mice (black bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) at 2 and 10 weeks after surgery, as assessed by immunohistochemical analysis. Mean ± SEM; *p < 0.05 vs. control. C Representative immunohistochemical images of MPO-positive cells (arrowheads) within the callus tissue of controls and PTH-treated mice at 2 and 10 weeks after surgery. Scale bars: 25 μm. D MPO-positive cells/HPF within the callus tissue of controls (white bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) and PTH-treated mice (black bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) at 2 and 10 weeks after surgery, as assessed by immunohistochemical analysis. Mean ± SEM; *p < 0.05 vs. control. E Representative immunohistochemical images of CD68-positive cells (arrowheads) within the callus tissue of controls and PTH-treated mice at 2 and 10 weeks after surgery. Scale bars: 25 μm. F CD68-positive cells/HPF within the callus tissue of controls (white bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) and PTH-treated mice (black bars, n = 5 at 2 weeks after surgery, n = 9 at 10 weeks after surgery) at 2 and 10 weeks after surgery, as assessed by immunohistochemical analysis. Mean ± SEM; *p < 0.05 vs. control