Fig. 4

Effect of BI-9740 (BI) on nitrotyrosine and poly(ADP-ribose) polymerase (PARP)-1 expression in grafts after heart transplantation (HTX). Representative images of A nitrotyrosine (× 100, scale bar: 500 µm) and B PARP-1 (× 400, scale bar: 100 µm) immunostaining are shown, along with semi-quantitative analysis of nitrotyrosine immunoreactivity (control: 8 rats, HTX: 10 rats, HTX + BI: 10 rats) and quantification of PARP-1-positive cells (control: 10 rats, HTX: 11 rats, HTX + BI: 10 rats). The samples were assessed by two examiners who were blinded to the experimental groups. The evaluation was conducted on 4–8 randomly selected non-overlapping fields per rat’s myocardial tissue. Values are expressed as mean ± SEM. *p < 0.05 vs. control, ^#p < 0.05 vs HTX