Fig. 4

Lycium barbarum glycopeptide induces microglia to secrete DHA. A Heat map of differential metabolic substances between the SCI and LbGp groups in spinal cord tissue(In vivo). B Heat map of differential metabolic substances between the ATP + LPS and LbGp treatment groups in microglial supernatants (In vitro). C Venn diagram of elevated metabolites in spinal cord tissues from the LbGp-treated group and microglia from the LbGp-treated group. D Microglia were activated with ATP + LPS for 4 hand incubated with LbGp for 24 h, and the DHA levels in the cell supernatants were assayed. E, F mRNA expression levels of FADS1 and FADS2 in ATP + LPS-stimulated microglia treated with LbGp. The data are presented as the means ± SEMs of at least 3 independent experiments; n = 6per group;*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 versus each group