Fig. 3

Lycium barbarum glycopeptide inhibits microglial MAPKs/NF-kB and pyroptosis-related pathways. A mRNA expression level of IL-18 in ATP + LPS-stimulated microglia treated with different doses(100 µg/kg–400 µg/ml) of LbGp. B–L The expression levels of P-p38, P38, p-JNK, JNK, P-p65, P-65, NLRP3, caspase-1-p20, ASC, GSDMD, GSDMD-N, IL-18, and IL-1β protein in each group of activated microglia were detected by western blotting. The data are presented as the means ± SEMs of at least 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 versus each group