Fig. 2

Lycium barbarum glycopeptide inhibits NF-kB-and pyroptosis-related proteins in vivo. A SCI treatment with different doses (10 mg/kg-100 mg/kg) of LbGp reduced the pro-IL-18 protein expression levels. B–D Fluorescence staining micrographs of NLRP3, ASC,andcaspase-1 protein on the 7th day in spinal cord tissues of the different groups. E–M On day 7, the protein expression levels of NLRP3, ASC, caspase-1, P-p65, P-65, GSDMD, GSDMD-N, IL-18, and IL-1β in spinal cord tissue from each group were detected by western blotting. The data are presented as the means ± SEMs of at least 3 independent experiments. Scale bar, 200 µm; n = 3 per group;*p < 0.05,**p < 0.01, ***p < 0.001, and ****p < 0.0001 versus each group