Fig. 6

LncRNA-Gm9866 affects liver fibrosis by interacting with Fam98b. A, B FISH assay shows the subcellular localization of lncRNA-Gm9866 in AML12 and JS-1 cells (magnification, 630X). C Ten putative lncRNA-Gm9866 target genes identified with the lncRRIsearch database. D, E mRNA expression levels of 10 predicted genes after overexpression and silencing of lncRNA-Gm9866 in AML12 cells were detected by qPCR. F Protein level of Fam98b following overexpressing and silencing of lncRNA-Gm9866 in AML12 cells assessed by western blot. G qPCR confirmation of Fam98b interference in AML12 cells. H mRNA expression level of lncRNA-Gm9866 in AML12 cells following knockdown of Fam98b expression. I FISH assay shows lncRNA-Gm9866 is co-localized with Fam98b in AML12 and JS-1 cells (magnification, 630X). J Fold-enrichment of lncRNA-Gm9866 in RNAs of AML12 cells combined with Fam98b. K Actinomycin D experiment shows lncRNA-Gm9866 had no effect on the mRNA stability of Fam98b in AML12 cells. L mRNA level of pro-fibrogenic and pro-apoptosis genes after silencing of Fam98b in stably transfected AML12 cells compared with cells overexpressing lncRNA-Gm9866. M Protein level of pro-fibrogenic and pro-apoptosis genes after silencing of Fam98b in stably transfected AML12 cells compared with AML12 cells overexpressing lncRNA-Gm9866. */^#P < 0.05, **/^##P < 0.01, ***/^###P < 0.001. *P < 0.05 vs. LV-control and ^#P < 0.05 vs. LV-Gm9866 in L. ns, no significance