Fig. 5
From: LncRNA-Gm9866 promotes liver fibrosis by activating TGFβ/Smad signaling via targeting Fam98b
Knockdown of lncRNA-Gm9866 attenuates CCl4-induced liver fibrosis in vivo. A qPCR shows that lenti-shGm9866 interferes with expression of lncRNA-Gm9866 in vivo. B The relative expression level of COL-1 in liver tissue increased significantly in NC + CCl4 group and decreased significantly in shGm9866 + CCl4 group. C The relative expression level of α-SMA in liver tissue increased significantly in NC + CCl4 group and decreased significantly in shGm9866 + CCl4 group. D Macroscopic observation showed that the liver appearance of shGm9866 + CCl4 group was significantly smoother than that of NC + CCl4 group. E H&E staining showed that the morphology of hepatocytes and the structure of hepatic lobules in shGm9866 + CCl4 group were more regular and regular than those in NC + CCl4 group. F Masson staining showed that the deposition of shGm9866 + CCl4 collagen fibers was less than that of NC + CCl4. G IHC results showed that positive expression of α-SMA and COL-1 was significantly elevated in the NC + CCl4 group and decreased significantly in the shGm9866 + CCl4 group. */# P < 0.05, **/##P < 0.01, ***/###P < 0.001. *P stands for NC + CCl4 vs NC or shGm9866 vs NC, #P stands for shGm9866 + CCl4 vs NC + CCl4