Fig. 3
From: LncRNA-Gm9866 promotes liver fibrosis by activating TGFβ/Smad signaling via targeting Fam98b
Knockdown of lncRNA-Gm9866 reduces TGFβ1-induced HCs apoptosis. A qPCR showed that knockdown of lncRNA-Gm9866 in AML12 cells abolished TGFβ1 (10 ng/mL, 24 h) induced upregulation of pro-fibrogenic genes α-SMA, COL-1, MMP9, and TIMP1 and apoptosis-related genes BAX and BAD. B, C Protein levels of α-SMA, COL-1, BAX, BAD, and Bcl2 were detected in lncRNA-Gm9866 knockdown and overexpressing AML12 cells by western blot. D mRNA levels of α-SMA, COL-1, MMP9, TIMP1, BAX, and BAD were detected in lncRNA-Gm9866-overexpressing AML12 cells by qPCR. E TUNEL assay indicated that overexpression of lncRNA-Gm9866 promoted apoptosis in AML12 cells (scale bar: 200 µm). */#P < 0.05, **/##P < 0.01, ***/###P < 0.001. *P < 0.05 vs. LV-control in D and *P < 0.05 vs. shRNA-control, #P < 0.05 vs. shRNA-control + TGFβ1 in A