Fig. 5

miR-134-5p regulated the expression of CREB1 by targeting it. A A Venn diagram was used to identify potential target genes of miR-134-5p in breast cancer, based on predictions from four online databases. B RT-qPCR was used to investigate the potential regulatory relationship between TDRKH-AS1 and four potential miR-134-5p target genes in breast cancer cells. C In silico prediction analysis suggested that miR-134-5p may bind to CREB1, a transcription factor that plays a role in regulating gene expression. D, E Luciferase reporter assay and RIP assay were used to confirm the interaction between miR-134-5p and CREB1 in breast cancer cells. F RT-qPCR was used to investigate the regulatory effect of miR-134-5p on CREB1 expression in breast cancer cells. G, H Western blot and RT-qPCR were used to investigate the potential clinical relevance of CREB1 as a target gene of miR-134-5p in breast cancer samples. ^∗P < 0.05; ^∗∗P < 0.01