Fig. 9

miR-100-5p relieves oxidative stress injury in MPTP-induced PD mice via the Nox4-ROS-Nrf2 axis. a Design and timeline of the animal experimental procedure. b Representational diagram of AAV injection site in mice and the co-labeling of TH with miR-100-5p showed that miR-100-5p was mainly expressed within the SNpc DA neurons. Scale bars, 100 µm; upper right, 10 µm. c–e Representative images and quantification of IHC of TH-positive neurons in the striatum and SNpc of the control, AAV-miR-NC, AAV-miR-100-5p, MPTP, MPTP + AAV-miR-NC, and MPTP + AAV-miR-100-5p groups (n = 3 per group). Scale bars, 1.000 mm for images in striatum; 500, 200, and 100 µm for the series of images in SNpc. f–g Immunofluorescence staining and quantification of TH expression in MPTP-stimulated PD mice after treatment with AAV-miR-NC or AAV-miR-100-5p (n = 3 per group). Scale bars, upper, 200 µm; lower, 100 µm. h–j Western blotting analysis showed the Nox4 and TH expression levels in the SN of the six groups (n = 3 per group). k–q Western blotting analysis showed the expression levels of PI3K, Keap1, Nrf2, HO-1, SOD-1, and SOD-2 in the SN of the six groups (n = 3 per group). The results are shown as mean ± SD. One-way ANOVA was used to analyze the data. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001