Fig. 8

miR-100-5p relieves oxidative stress injury in MPP⁺-induced MN9D cells via the Nox4-ROS-Nrf2 axis. a–c Representative blots and quantification showed the levels of Nox4 and TH in control and MPP⁺-induced MN9D cells after miR-100-5p inhibitor/mimics transfection. d–j Western blotting analysis of PI3K, Keap1, Nrf2, HO-1, SOD-1, and SOD-2 in control or MPP⁺-induced MN9D cells after miR-100-5p inhibitor/mimic transfection. Each experiment was independently repeated three times. The results are shown as mean ± SD. One-way ANOVA was used to analyze the data. *p < 0.05, **p < 0.01, ns no significant