Fig. 4

Sequencing analysis of T-MSCs-Exo miRNAs, and miR-100-5p directly targets the 3' UTR NOX4. a Size distribution of miRNA reads was between 18 and 26 nt with a peak of 23 nt. b Summary of known and predicted miRNAs. c KEGG pathway analysis. d, e qRT-PCR assay of miR-100-5p and NOX4 expression after T-MSCs-Exo treatment of control or MPTP-induced PD mice. f, g qRT-PCR assay of miR-100-5p and NOX4 expression in control or MPP⁺-induced MN9D cells after miR-100-5p inhibitor/mimic transfection. h Wild-type and mutated-type binding sites between miR-100-5p and NOX4. i Dual luciferase reporter assay of MN9D cells in the presence of indicated treatments. Each experiment was independently repeated three times. The results are shown as mean ± SD. One-way ANOVA was used to analyze the data. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and ns: no significant