Fig. 5

Function of signature genes of E. coli sepsis in related cell clusters. A-C Pseudotime analysis of monocyte cell clusters. Trajectory of monocyte cell clusters is inferred using monocle2 and clusters are marked by colors A Pseudotime-ordered variables are inferred B Cells derived from case or control are displayed separately on the differentiation trajectory C Lines and arrows indicate inferred differentiation trajectory and direction. D Heatmap showing the expression of genes related cell fate decision. E Functional enrichment of genes related to two differentiation trajectories. F The bar chart shows the relative cell abundance of C2_C1QA (left) and C10_ULK1 (right) in WT and KO mice. G Expression of DRAM1 in C10_ULK1 between sepsis and controls. The P value is from a Wilcoxon test. H and I GSEA of autophagy-related genes in C10_ULK1 H and GSE4607 dataset I, comparing controls and septic patients. Nominal P value and the false-discovery rate (FDR) are indicated. J Schematic diagram of monocyte cluster differentiation