Fig. 4

The related cell clusters of E. coli sepsis. A The UMAP plot shows the clustering of 152,636 cells from 69 samples into 18 clusters. B Cell clusters are defined by a set of known marker genes. C The density scatter plot shows the expression levels of the E. coli sepsis signature. The color gradient represents the enrichment score, yellow indicates a higher score. D The density heatmap shows the expression and distribution of the E. coli sepsis signature in different cell subclusters. The color gradient represents the enrichment score, red indicates a higher score. E The heatmap shows the statistical significance of the E. coli sepsis signature in each cell subcluster as determined by the RRA method. The top bar graph shows the different cell subclusters, and the bottom bar graph shows the upregulation or downregulation of the signature gene set in each subcluster. F The marker genes of each monocyte cluster. G The bar chart shows the relative cell abundance of C1_CD36 and C10_ULK1 in controls and septic patients. P values are from a Wilcoxon test