Fig. 4

Verification of the circGLIS3/miR-1343-3p/PGK1 axis by the HGC27 cell line in a rescue experiment. A, B. The expression levels of PGK1 mRNA and protein and the AKT signaling pathway were measured by qRT‒PCR and WB after simultaneous knockdown of circGLIS3 and miR-1343-3p in the HGC27 cell line. Cotransfection of miR-1343-3p inhibitor and circGLIS3 siRNA to investigate malignant transformation of cells with Transwell (C), wound healing (D), and EdU assays (E) in the HGC27 cell line* P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001