Fig. 3

Overexpression of HOTAIRM1 promotes glioma tumor growth and VM formation in the xenograft mouse model. A Representative images of tumors collected from mice injected with U251 cells transfected with over- HOTAIRM1, si-HOTAIRM1or control. B Tumor volume was measured every 7th day, and tumor weight was detected on day 28 after injection, respectively. C Expression level of Ki67 in xenograft tissues from different treatment groups (Scale bar = 200 μm). D TUNEL assay was performed to detect cell apoptosis in different treatment groups (magnification, × 200). E CD34-PAS immunohistochemistry was used to assess glioma cell VM density affected in indicated treatment groups (Scale bar = 100 μm). Data are presented as the mean ± SD of three independent experiments (n = 3). **P < 0.01, ***P < 0.001