Fig. 6

USP39 promotes SIRT7 expression by activating the transcriptional activity of FOXM1. A Western blot was used to detect the protein expression of FOXM1 in the cytoplasm and nucleus of CaSki and SiHa cells transfected with shUSP39#1/#2. B qRT-PCR was used to measure the mRNA expression of SIRT7 after overexpressing FOXM1 in CaSki and SiHa cells. C ChIP assay was performed to explore the interaction between FOXM1 and SIRT7 in CaSki and SiHa cells. D Dual-luciferase reporter assays were conducted to measure the luciferase activity of the pGL3-SIRT7 promoter in CaSki and SiHa cells after indicated transfection. All results are representative of at least 3-independent experiments. **p < 0.01, ***p < 0.001; ###p < 0.001