Fig. 5

USP39 regulates alternative splicing of FOXM1 pre-mRNA. A qRT-PCR and western blot analyses were performed to detect the mRNA and protein levels of SIRT7 in CaSki and SiHa cells transfected with shUSP39#1/#2. B The correlation of USP39 and FOXM1 in the cervical squamous cell carcinoma tissues was predicted using the GEPIA database (http://gepia.cancer-pku.cn/). GEPIA database sources CESC tumor and CESC normal samples in the TCGA database. C Western blot was used to detect FOXM1 protein levels in CaSki and SiHa cells after USP39 knockdown. D qRT-PCR analysis was used to measure the mRNA expression of spliced and unspliced FOXM1 RNA transcripts and (E) Splicing efficacy in CaSki and SiHa cells transfected with shUSP39#1/#2. F The interaction between FOXM1 and USP39 was explored using RIP assay. G qRT-PCR was used to evaluate the overexpression efficacy of FOXM1 in CaSki and SiHa cells. H The expression of SIRT7 in cervical squamous cell carcinoma cells after indicated transfections were measured using qRT-PCR analysis. All results are representative of at least 3-independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001