Fig. 4

USP39 promotes tumorigenesis in CSCC. A Western blot was used to detect the protein expression of USP39 in tumor tissues and adjacent normal tissues of cervical squamous cell carcinoma patients. B The protein level of USP39 in human cervical squamous cell carcinoma cell lines (CaSki, SiHa) and human normal cervical epithelial cells (Ect1/E6E7) was detected using western blot assay. C IHC assay was performed to detect the protein expression of USP39 in the normal and tumor tissues of cervical squamous cell carcinoma patients. D The correlation analysis was performed between the expression of SIRT7 and USP39 in the tumor tissues of cervical squamous cell carcinoma patients. E The knockdown efficiency of USP39 in CaSki and SiHa cells was assessed using qRT-PCR analysis. F Colony formation and (G) CCK-8 assays were conducted to evaluate the proliferation potential and viability of CaSki and SiHa cells transfected with shUSP39#1/#2. H The apoptosis rate of CaSki and SiHa cells after silencing USP39 was measured by flow cytometry. All results are representative of at least 3-independent experiments. ***p < 0.001