Fig. 3

SIRT7 interacts with and mediates USP39 deacetylation. A IF staining was used to detect the subcellular location of SIRT7 and USP39 in CaSki and SiHa cells. B Co-IP assays were conducted to evaluate the interaction between USP39 and SIRT7 in CaSki and SiHa cells. C USP39 acetylation levels were examined by IP-WB in CaSki and SiHa cells treated with NAM or TSA inhibitors. D IP-WB was used to detect the acetylation level of USP39 in SIRT7 silenced CaSki cells. E The USP39 acetylation levels in CaSki cells with wild-type or mutant SIRT7 (SIRT7 H187Y) were detected by WB. F Sequence alignment of conserved K428-containing regions in USP39 orthologs of multiple species. G IP-WB assays were used to determine the acetylation level of USP39 in CaSki cells with wild-type or mutant USP39. H Western blot was performed to measure the protein expression of USP39 in CaSki cells after CHX treatment in indicated groups. All results are representative of at least 3-independent experiments. **p < 0.01