Fig. 2

SIRT7 knockdown inhibits autophagy to promote ROS accumulation. A Western blot was used to detect the key proteins related to autophagy (LC3-I, LC3-II) in CaSki and SiHa cells after transfection of shSIRT7#1/#2. B TEM was used for the observation and quantification of autophagosomes in each group. C The mRFP-GFP-LC3 adenovirus infection assay was performed to examine the autophagy in CSCC cells. D JC-1 staining assay was used to detect the mitochondrial membrane potential of CSCC cells after indicated transfection. E DCFH-DA staining was used to detect the ROS levels in CSCC cells after indicated transfection. All results are representative of at least 3-independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001