Fig. 1

SIRT7 promotes cervical squamous cell carcinoma tumorigenesis both in vitro and in vivo. A The expression pattern of SIRT7 in cervical squamous cell carcinoma (CSCC) (n = 305) and normal tissue (n = 3) was predicted using the UALCAN database (http://ualcan.path.uab.edu/). B SIRT7 mRNA levels in the tumor tissues (n = 40) of CSCC patients and normal adjacent tissues (n = 40) were measured using qRT-PCR analysis. C IHC analysis was performed to determine the protein levels of SIRT7 in CSCC tissues and normal tissues. D qRT-PCR and western blot analysis were used to detect the mRNA and protein expression levels of SIRT7 in human cervical squamous cell carcinoma cell lines (CaSki, SiHa, C-33A) and human normal cervical epithelial cells (Ect1/E6E7 cells). E SIRT7 expression in CaSki and SiHa cells after the transfection of shSIRT7#1/#2 was measured using qRT-PCR. F Colony formation assay was conducted to evaluate the proliferation of CSCC cells after silencing SIRT7. G CCK-8 assay was used to detect the viability of CSCC cells transfected with shSIRT7#1/#2. H Flow cytometry was used to assess the apoptosis rate of CSCC cells after SIRT7 knockdown. I Tumor volume of mice injected with transfected SiHa cells (shNC or shSIRT7#1) (n = 6 per group). J The images of mouse tumors and the tumor weight in the shNC and shSIRT7 groups. All results are representative of at least 3-independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001