Fig. 5

Neuroinflammatory component in cerebral cortex of symptomatic Rett mice is corrected after leriglitazone treatment. A Rett cerebral cortex at 7 m.o. showed a pattern of increased cytokines expression detected by flow cytometry, that was corrected in leriglitazone-treated mice (Nested one-way ANOVA analysis; p-value Rett vs wt = ** 0.0016; Rett vs Rett + leriglitazone = **** < 0.0001) B Iba1 and D GFAP cell-positive number were appraised, as microglia and astrocytic specific markers, respectively. C, E Quantifications of Iba1- and GFAP-positive cells revealed an increase in microglia and active astrocytes in symptomatic Rett mice cortex compatible with neuroinflammation, that was corrected with LGZ for both markers. Images were taken at a 10 × magnification (and 2.5 × electronic zoom for Iba1 images). Scale bar represents 50 µm. Statistical analyses were performed as described in Materials and Methods. *p < 0.05, **p < 0.01. Absence of asterisk means no statistically significant difference. Wild-type female mice in grey; Rett female mice in yellow; LGZ-treated Rett female mice in green