Fig. 2

Bioenergetic characterization of pre-symptomatic (3 m.o.) and symptomatic (7 m.o.) Rett mice. Given the time-dependent character of Rett syndrome, we studied both wild-type and Rett mice at two development time points: A pre-symptomatic (3 months) and B post-symptomatic (7 months) brains. At the time points, mice brains were dissected and subjected to analysis. ATP concentration by luciferin-luciferase luminescence and lipid peroxidation by TBARS were analysed in three brain areas: cortex, hippocampus and cerebellum. All experiments were done in at least three different mice, with three technical replicates. The ATP production experiment was done in triplicates, while lipid peroxidation was a unique experiment. Statistical analyses were performed as described in Materials and Methods. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Absence of asterisk means no statistically significant difference. Wild-type female mice in grey; Rett female mice in yellow