Fig. 7

EGF stimulation of DLD1 transfected cells. A Quiescent DLD1-pEGFP and DLD1-pEGFP_hRIZ2 were untreated or treated with exogenic hEGF at short time points (5-, 10- and 30 min). EGFR and ERK were analyzed by Western blots, which are representative of three different experiments. Expression levels of proteins were analyzed by densitometry analysis, using NIH Image J Software. The ratio between pEGFR/EGFR (left graph) and pERK/ERK (right graph was evaluated. Results were expressed as relative ratio. Means and SDs are shown. B The effect of ZD1839 on EGFR and ERK phosphorylation in EGF stimulated cells at 5 min (DLD1-pEGFP_hRIZ2 cells) and 10 min (DLD1-pEGFP cells). Phosphorylation of FAK was also evaluated. Densitometric analysis was performed as in A. C DLD1-pEGFP and DLD1-pEGFP_hRIZ2 quiescent cells were wounded and left untreated or treated for 30 h in absence or presence of EGF and ZD1839, as indicated in the figure. Wound area was calculated using Leica Suite software. Data are presented as the percentage of residual area in wound width over the control cells, analyzed at time 0. Means and SDs are shown. Pictures are representative of three different experiments. ****p < 0.0001; ns not significant