Fig. 9

RAC1 protein and mRNA expression increases in HCC cells after treatment with tigecycline. RAC1 protein expression in Huh7 (A, C) and HepG2 (B, D) after treatment with 10 µM (light blue bar graphs) and 20 µM (dark blue bar graphs) tigecycline for 24 h (A, B) and 48 h (C, D); E RAC1 mRNA expression in Huh7, HepG2, and THLE-2 after treatment with 10 µM (light blue bar graphs) and 20 µM (dark blue bar graphs) tigecycline for 24 h. Bar graphs represent the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001; ns = no significance compared with the control group (grey bar graphs)