Fig. 7

Bufalin reversed tumor‑promoting effect induced by CAMKK2 in vivo. A Tumor-bearing mice and the dissected tumors were photographed and shown. B A ruler was used to indicate the size of the tumors. C Statistics and comparison of tumor volume in overexpression group, bufalin treatment group and vector. D HE staining was used to detect the tumors. E HE staining of main organs in nude mice. Representative HE staining photos of heart, liver, spleen, lung and kidney of each group of nude mice. Bufalin had no obvious side effects on heart, liver, spleen, lung and kidney of nude mice. Protein levels were normalized to tubulin. All results were presented as the mean ± SD (n = 3). * p < 0.05, **p < 0.01