Fig. 6

Bufalin inhibits CAMKK2-induced Wnt/β-catenin signaling pathway in vitro and vivo. A Western blot analysis of expression of proteins related to Wnt/β-catenin signal pathway in HCCC-9810 and QBC-939 cells overexpressing CAMKK2 or not, exposed to bufalin or not. B The quantification of western blot (A). Protein levels were normalized to tubulin. C Western blot analysis of expression of proteins related to vimentin, N-cadherin and Wnt/β-catenin signal pathway in solid tumors. D The quantification of western blot (C). Protein levels were normalized to tubulin. All results were presented as the mean ± SD (n = 3). * p < 0.05, **p < 0.01