Fig. 5

Bufalin reversed the metastatic promotion induced by CAMKK2 in vitro. A Western blot analysis of expression of Flag-CAMKK2 protein in HCCC-9810 and QBC-939 cells overexpressing CAMKK2 or vector. B CCK8 assay in HCCC-9810 and QBC-939 cells overexpressing CAMKK2 exposed to bufalin or vector. C Would healing in HCCC-9810 and QBC-939 cells overexpressing CAMKK2 exposed to bufalin or vector. D Statistical analysis of the cell migration in the wound healing assays. E Transwell in HCCC-9810 and QBC-939 cells overexpressing CAMKK2 exposed to bufalin or vector. F Statistical analysis of the cell migration in Transwell. G Western blot analysis of expression of metastasis related proteins N-cadherin and Vimentin in HCCC-9810 and QBC-939 cells. H The quantification of western blot (F). Protein levels were normalized to tubulin. All results were presented as the mean ± SD (n = 3). * p < 0.05, **p < 0.01