Fig. 4

SKL2001 reversed the inhibitory effect of bufalin on the proliferation and migration of ICC. A In HCCC-9810 and QBC-939 cells, SKL2001 was activated after treatment with bufalin to detect the migration ability of the cells. After activation of SKL-2001, the cells were treated with bufalin to detect their ability to migrate. B above S indicates treatment with bufalin first, and S above B indicates treatment with advanced SKL-2001 activator. B Statistical analysis of (A) wound healing experiment. C In HCCC-9810 and QBC-939 cells, SKL2001 was activated after treatment with bufalin, and the expression levels of vimentin, N-cadherin, β-catenin and CCND1 were detected. D Statistical analysis of protein level of C. E The expression levels of vimentin, N-cadherin, β-catenin and p-β-catenin were detected in HCCC-9810 and QBC-939 cells after activation of SKL-2001 and treated with bufalin. F Statistical analysis of protein level of E. All results were presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01