Fig. 3

Bufalin inhibited Wnt/β-catenin signal pathway in ICC cells. A Western blot analysis of expression of proteins related to Wnt/β-catenin signal pathway in HCCC-9810, RBE and QBC-939 cells treated with bufalin or control. B The quantification of western blot. Protein levels were normalized to tubulin. C-E Cell localization and expression levels of β-catenin, CCND1 and p-β-catenin. All results were presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01