Fig. 3
From: CRISPR/Cas9 technology: applications in oocytes and early embryos
Diagram of transcriptional regulation and protein labeling by CRISPR/Cas9 system. a Diagram showing that the inactive Cas9 protein can be fused to epigenetic modifiers like DNMT3A and TET1 to alter gene expression by modifying the methylation state of cytosine in the specific promoter. b Diagram of pronuclear injection process to generate reporter mice. The RNP complex which contained Cas9 protein, crRNA and tracrRNA, and Pax6-IR4ES-EGFP targeting vector was injected into mouse fertilized eggs to obtain knock-in mice