Fig. 8

NT-3 activates ERK pathway signaling through TrkC in Schwann cells. a Representative images of the distal common peroneal nerve after 1 week of denervation by immunostaining with S100β and TrkC. Scale bar, 30 μm. b Western blotting used to detect the expression of TrkC in the injured distal stumps after 1, 3, 5, 8, and 10 weeks of denervation. c Relative quantitative analysis of TrkC expression. Data were normalized to 1 week. One-way ANOVA with Dunnett’s multiple comparisons test, n = 3. d Representative images of Schwann cells (passage 2) by immunostaining with S100β and TrkC, scale bar, 50 μm. e Western blot analysis of TrkC expression in Schwann cells at different passages (passages 2, 4, 6, 8). f Relative quantitative analysis of TrkC expression. Data were normalized to passage 2. There is no statistically significant difference. One-way ANOVA with Dunnett’s multiple comparisons test, n = 3. g Western blot analysis of ERK, p-ERK, and c-Jun expression in the control, NT-3, NT-3 + DMSO, and NT-3 + K252a groups. h, i Relative quantitative analysis of the expression of p-ERK/ERK and c-Jun show that K252a prevents the phosphorylation of ERK and the upregulation of c-Jun caused by NT-3. The data were normalized to the control. One-way ANOVA with Tukey’s multiple comparison test, **P < 0.005, ***P < 0.001, n = 3 or 4. j Western blot analysis of TrkC, ERK, p-ERK, and c-Jun expression in the si-control, si-TrkC (1), and si-TrkC (2) groups. k–m Relative quantitative analysis of the expression of TrkC, p-ERK/ERK, and c-Jun. The data were normalized to the control. One-way ANOVA with Tukey’s multiple comparison test. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001, n = 3. All numerical data are presented as mean ± SEM