Fig. 4

Reinnervation of target muscles by regenerated axons. a Representative images of NMJs using immunofluorescence staining. Green: Presynaptic NMJs and regenerated axons. Red: AChRs in the post-synapses of NMJs; scale bar, 100 μm. b Percentage of reinnervated NMJs per group, n = 12 from 3 rats. c the diameter of the anterior tibial muscle fibers in each group was shown by H&E staining, scale bar, 50 μm. d The proportion of the wet weight of the tibialis anterior muscle in each group, n = 3 or 4. e Representative immunofluorescence images of FITC-CTB-labeled positive sensory neurons in the DRG; scale bar, 200 μm. f, g Percentages of FITC-CTB-labeled sensory and motor neurons in the DRG and anterior horn of the spinal cord. n = 3 or 4. All numerical data were analyzed using one-way ANOVA with Tukey’s multiple comparison tests and are represented as mean ± SEM. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001